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Kamiya, Tomihiro; Hirao, Toshio; Kobayashi, Yasuhiko
Nuclear Instruments and Methods in Physics Research B, 219-220, p.1010 - 1014, 2004/06
Times Cited Count：8 Percentile：48.81(Instruments & Instrumentation)no abstracts in English
)Asaka, Tomomi*; Hashimoto, Chika*; Ito, Kazusa*; Yasuda, Takako*; Nagata, Kento*; Nishimaki, Toshiyuki*; Katsumura, Takafumi*; Ota, Hiroki*; Suzuki, Michiyo; Funayama, Tomoo; et al.
no journal, ,
It is thought that the irradiation to the particular area of the individual could induce the irradiation impact to the outside of the irradiated area in addition to the irradiated region. In this study, we established the procedures to conduct carbon ion irradiation on adult Medaka, which can irradiate only either dorsal or ventral side of the fish, using the broad-beam irradiation system of JAEA-Takasaki. The irradiated fish were examined heart rate before the fixation. Thereafter, the systemic alteration was evaluated by serial cross sections of the whole body of the fish. Tissues at the irradiated side were seriously injured that corresponded to the range of the beam. However, secondary gill lamella showed the characteristic edema in either the ventral or dorsal irradiated fish unless the gill located at ventral, suggesting the systemic irradiation effect. There was no alteration in the heart rate after the irradiation suggesting no severe effects in blood flow in this condition.
Ueda, Daisuke*; Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Shirai, Koji*
no journal, ,
The occurrence of DNA damage-induced cell cycle arrest is consider to depend on the amount and/or seriousness of DNA damage. However, the detailed mechanisms have been obscure. When whole egg of the silkworm in early embryogenesis was subjected to carbon ions, the developmental arrest was observed, however, about 2 hours after irradiation, the mitotic cleavage resumed, indicating the eggs at this stage can induce the cell cycle arrest by checkpoints after irradiation. On the other hand, the locally targeted irradiation with carbon-ion microbeam to 10% of the nuclei was not able to induce the developmental arrest. In the egg, several of abnormal cleaved nuclei were observed, which are considered to be the irradiated nuclei that stop their cleavage. Meanwhile, when 30-40% of nuclei were irradiated, the embryonic development stopped. The result indicate the checkpoint-induced arrest of the silkworm egg at intralecithal cleavage stage may depend on number or ratio of the damaged nuclei.
Oda, Shoji*; Yasuda, Takako*; Hibi, Yusuke*; Asaka, Tomomi*; Ikeda, Hiroko; Muto, Yasuko*; Yokota, Yuichiro; Sakashita, Tetsuya; Suzuki, Michiyo; Funayama, Tomoo; et al.
no journal, ,
We have developed a new irradiation protocol for tissue specific irradiation to living embryo and adult medaka, . In the study, a testis of p53 knockout fish were irradiated with multi-shots of microbeam. Less numbers of testis-ova were induced in the in p53- lacking testis 1 week after the irradiation compared to whole body irradiated testis. Specific irradiation of microbeam onto the right (or left) lobe of the optic tectum in medaka embryos of 2 days post fertilization ectopically induced apoptotic cells in the body trunk and the tail in addition to the irradiated position. The systemic effects as endocrine system, autonomic nervous system and immunity reactions occur in adult medaka and these results suggested that the systemic effects might be functional even in developing embryos.
Narukawa, Takafumi; Yamaguchi, Akira*; Jang, S.*; Amaya, Masaki
no journal, ,
no abstracts in English
